| Czech Republic MotoGP, Brno - Qualifying Results | ||||
| Pos | Rider | Nat | Team | Lap |
| 1 | Marc Marquez | SPA | Repsol Honda RC213V | 2m 02.753s |
| 2 | Jack Miller | AUS | Pramac Ducati GP19 | 2m 05.277s |
| 3 | Johann Zarco | FRA | Red Bull KTM Factory RC16 | 2m 05.361s |
| 4 | Andrea Dovizioso | ITA | Mission Winnow Ducati GP19 | 2m 05.590s |
| 5 | Pol Espargaro | SPA | Red Bull KTM Factory RC16 | 2m 05.710s |
| 6 | Alex Rins | SPA | Suzuki Ecstar GSX-RR | 2m 06.172s |
| 7 | Valentino Rossi | ITA | Monster Yamaha YZR-M1 | 2m 06.233s |
| 8 | Danilo Petrucci | ITA | Mission Winnow Ducati GP19 | 2m 06.457s |
| 9 | Maverick Viñales | SPA | Monster Yamaha YZR-M1 | 2m 06.626s |
| 10 | Fabio Quartararo | FRA | Petronas Yamaha YZR-M1* | 2m 06,.648s |
| 11 | Cal Crutchlow | GBR | LCR Honda RC213V | 2m 07.123s |
| 12 | Franco Morbidelli | ITA | Petronas Yamaha YZR-M1 | 2m 09.404s |
| Positions 13 to 23 decded in Q1 | ||||
| 13 | Takaaki Nakagami | JPN | LCR Honda RC213V | 2m 05.033s |
| 14 | Francesco Bagnaia | ITA | Pramac Ducati GP18* | 2m 05.053s |
| 15 | Sylvain Guintoli | FRA | Suzuki Ecstar GSX-RR | 2m 05.265s |
| 16 | Aleix Espargaro | SPA | Factory Aprilia Gresini RS-GP | 2m 05.286s |
| 17 | Stefan Bradl | GER | Repsol Honda RC213V | 2m 05.763s |
| 18 | Miguel Oliveira | POR | Red Bull KTM Tech3 RC16* | 2m 05.974s |
| 19 | Joan Mir | SPA | Suzuki Ecstar GSX-RR* | 2m 06.554s |
| 20 | Hafizh Syahrin | MAL | Red Bull KTM Tech3 RC16 | 2m 06.898s |
| 21 | Karel Abraham | CZE | Reale Avintia Ducati GP18 | 2m 07.155s |
| 22 | Tito Rabat | SPA | Reale Avintia Ducati GP18 | 2m 07.315s |
| 23 | Andrea Iannone | ITA | Factory Aprilia Gresini RS-GP | 2m 07.923s |
