A statement also confirmed that the Italian will not be replaced, meaning Maverick Vinales will be the team's only rider this weekend.
"The whole of Yamaha wishes Valentino Rossi a speedy recovery and hopes to see him back on his YZR-M1 as soon as possible," the statement added.
The statement said: 'The Movistar Yamaha MotoGP Team regrets to report that Valentino Rossi will not be able to compete in the upcoming Gran Premio Tribul Mastercard di San Marino e della Riviera di Rimini, to be held this weekend.
'The Factory Yamaha team will compete in round 13 of the 2017 MotoGP World Championship with Maverick Viñales as its sole rider.
'The whole of Yamaha wishes Valentino Rossi a speedy recovery and hopes to see him back on his YZR-M1 as soon as possible.'
Doctors predict Rossi will need 30-40 days before returning to MotoGP action, after undergroing surgey on on a broken leg. That would mean missing Misano and Aragon before a comeback at Motegi. But much depends on the speed of Rossi's recovery and Aragon (22 days) remains a possibility...
Factory test rider Katsuyuki Nakasuga would be an obvious replacement, should the nine-time world champion not ride in Aragon. Nakasuga's Suzuka team-mate and Yamaha WorldSBK star Alex Lowes also has recent MotoGP experience from stand-in rides last year, but would need permission from Yamaha Europe.
Rossi is currently 26 points from Ducati's title leader Andrea Dovizioso. Vinales is third in the standings, 13 points from the Ducati rider.
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